• Diet 4 MicroGut

    Microorganisms in foods and in humans: study of the microbiota and the related metabolome.

  • Diet 4 MicroGut

    Microorganisms in foods and in humans: study of the microbiota and the related metabolome.

  • Diet-Human health

    The human body houses a huge microbial ecosystem, including the intestinal and oral microbiota. Both these ecosystems, and, in particular, the intestinal one, are responsible for maintaining human health.

  • Diet-Microbiota relationship

    Diet is a reservoir of microbes and the feeding for human resident microorganisms, therefore, it is inevitably linked to diversity and functionality of the oral andintestinal microbiota.

  • Few information is available

    A cross-search for "human gut microbiota" and "health" in the ISI Web of Knowledge database, results in more than 550 hits, only in the last 5 years. Less than 10 hits come out combining the terms “vegetarians” and "human gut microbiota"

Created on 08 September 2015

In this study, the fecal microbiota of 153 healthy volunteers, recruited from four different locations in Italy, has been  studied by coupling viable counts, on different microbiological media, with ribosomal RNA Denaturing Gradient Gel Electrophoresis (rRNA-DGGE). The volunteers followed three different diets, namely omnivore, ovo-lacto-vegetarian and vegan. The results obtained from culture-dependent and -independent methods have underlined a high level of similarity of the viable fecal microbiota for  the three  investigated diets. The rRNA DGGE profiles were very complex and comprised a total number of bands that varied from 67 to 64 for the V3 and V9 regions of the 16S rRNA gene, respectively. Only a few bands were specific in/of all three diets, and the presence of common taxa associated with the dietary habits was found. As far as the viable counts are concerned, the high similarity of the fecal microbiota was once again confirmed, with only a few of the investigated groups showing  significant differences. Interestingly, the samples grouped differently, according to the recruitment site, thus highlighting a higher impact of the food consumed by the volunteers in the specific geographical locations than that of the type of diet. Lastly, it should be mentioned that the fecal microbiota DGGE profiles obtained from the DNA were clearly separated from those produced using RNA, thus underlining a difference between the total and viable populations in the fecal samples.

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